ABSTRACT
OBJECTIVE: To study the basic characteristics of three bioactive fungi, the interrelationship of three fungi and the relationship between three fungi and their host, thus to provide data for the theoretical research and the application practice. METHODS: The colonial morphology and microscopic characteristics were studied by light microscopy, and the relationships were studied by agar diffusion method. RESULTS: The basic characteristics of three bioactive fungi were obtained, and the relationships under in vitro conditions were clarified. CONCLUSION: The results of this study will be of great value to the studies and use of the fungi, and it will provide important data for research on the mechanism of fungi inducing Aquilaria sinensis to produce Agilawood.
ABSTRACT
OBJECTIVE: To optimize the solid culture condition of three fungi which can induce Aquilaria sinensis to produce agilawood, thus to provide data for the production and application of the fungi. METHODS: Single factor experiment method was applied in the study. RESULTS: The optimal solid culture medium formula, temperature, pH value, moisture, the density of materials and illumination conditions for three fungi were obtained. CONCLUSION: The optimized method can provide high quality fungi for prompting Aquilaria sinensis to produce agilawood at larger scales. Copyright 2012 by the Chinese Pharmaceutical Association.
ABSTRACT
OBJECTIVE: To investigate the feasibility of utilizing three fungi YNAS04, YNAS06 and YNAS08 to induce the formation of agilawood from Aquilaria sinensis in field and laboratory, respectively. METHODS: Agarwood was obtained by inoculating the fungus in the stem of A. sinensis under natural conditions, and the main ingredients of the volatile oil from induced agilawood were analyzed by GC-MS. The authenticity of the used fungi was detected by the method of morphology and ribosomal internal transcribed spacer. RESULTS: The weights of the induced materials from YNAS04, YNAS06 and YNAS08 were (1.786±0.473), (2964±0.492) and (3.615±0.591) g after six months, which were heavier than blank control (1.325±0.407) g and negative control (1.462±0.417) g, respectively. The fungus inoculated in the stem of A. sinensis and became the dominant fungi in the tissue under natural conditions. Sesquterpenoids and chromone analogues were detected from three fungi inducing materials of six months old. CONCLUSION: Utilizing fungi to induce the formation of agilawood from Aquilaria sinensis has the advantages of spending less time and producing high-quality secondary metabolites. This method has important application foreground in large-scale agilawood production.